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Bacterial Artificial Chromosomes Volume 1 Library Construction Physical Mapping and Sequencing Methods in Molecular Biology From the Back Cover The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes expert investigators describe not only the classic methods but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 Library Construction Physical Mapping and Sequencing presents readily reproducible techniques for library construction physical mapping and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format each one offering step-by-step laboratory instructions an introduction outlining the principle behind the technique lists of equipment and reagents and tips on troubleshooting and avoiding known pitfalls. Besides protocols each chapter includes scientific reviews software tools database resources genome sequencing strategies and illustrativ
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